development of a multiplex pcr assay for detection and

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Author Li Zhao, Guixia Li, Ji Wang, Mengchuan Zhao, Le Wang, Zhishan Feng, Xuejun MaPublish Year 2020(plate) Author Xiaofeng Mu, Xiaofeng Mu, Wen Chen, Junying Shi, Xinmin Li, Ye WangPublish Year 2019Development of a Molecular Beacon-based Multiplex Real development of a multiplex pcr assay for detection and

Non Technical Summary As most C. perfringens gastrointestinal pathogens cause very similar clinical symptoms, differential diagnosis of the pathogens is required in one sample. This project will develop a rapid and highly reliable molecular beacon-based multiplex real-time PCR assay for the detection of Clostridium perfringens infections in domestic animals.(plate) Cited by 11Publish Year 2014Author D.F. Zhang, D.F. Zhang, Q.Q. Zhang, A.H. LiDevelopment of a multiplex PCR assay for simultaneous development of a multiplex pcr assay for detection and(steel) This study describes development and evaluation of multiplex and single PCR assays for simultaneous detection of Theileria annulata, Babesia bovis and Anaplasma marginale in cattle.

Cited by 12Publish Year 2014Author Abdelfattah M. Selim, Mahmoud M. Elhaig, Wolfgang GaedeDevelopment of a multiplex real-time PCR assay for development of a multiplex pcr assay for detection and

Detection of Clinical Specimens by the GeXP Multiplex PCR Assay. Using the optimized GeXP multiplex PCR assay, the expression of 8 kinds of lncRNAs was detected in the 23 pairs consisting of specimens with HCC and their adjacent noncancerous counterparts. The results of GeXP multiplex PCR assay are shown in Figure 3.(plate) Cited by 15Publish Year 2017Author Hansong Chae, Seung Jung Han, Su Young Kim, Chang Seok Ki, Hee Jae Huh, Dongeun Yong, Won Jung Koh, development of a multiplex pcr assay for detection andDevelopment and evaluation of a multiplex PCR assay for development of a multiplex pcr assay for detection and(steel) Multiplex PCR is a powerful tool in clinical microbiology and has been widely applied to detect bacteria and genes of interest (Kong et al. 2002). The current report describes the development and evaluation of a novel mPCR assay for rapid detection of 11 important VAGs of P. multocida isolates from pigs. The mPCR assay is rapid and takes development of a multiplex pcr assay for detection and(plate) Cited by 1Publish Year 2019Author Seok Hwee Koo, Yee Leng Teoh, Wei Liang Koh, Harumi Ochi, Sher Kye Tan, Diana Miao Fang Sim, Boran J development of a multiplex pcr assay for detection andThe development of a multiplex PCR assay for the detection development of a multiplex pcr assay for detection and(steel) Aug 02, 2020The development of a multiplex PCR assay for the detection of Fusarium oxysporum f. sp. cubense lineage VI strains in East and Central Africa P. Ndayihanzamaso 1 P. Karangwa 1 , 2

Cited by 26Publish Year 2012Author Douglas J. Botkin, Lucía Galli, Vinoth Sankarapani, Michael Soler, Marta Rivas, Alfredo G. TorresDevelopment of two real-time multiplex PCR assays for

The aim of this study was to develop and evaluate two multiplex real-time PCR assays for the sensitive detection and accurate quantication of Streptococcus pneumoniae, Haemophilus inuenzae, Staphylococcus aureus, Moraxella catarrhalis, Escherichia coli, Klebsiella(plate) Cited by 27Publish Year 2015Author Liu Junlong, Youquan Li, Aihong Liu, Guiquan Guan, Junren Xie, Hong Yin, Jianxun LuoDevelopment of a Multiplex PCR Assay for Detection and development of a multiplex pcr assay for detection and(steel) Jan 01, 2012A multiplex PCR-based assay for N. meningitidis detection and genogrouping was developed based on screening out specific genes and primers for each N. meningitidis serogroup. This assay was tested on 179 bacterial isolates and proved to be specific, accurate, and sensitive.(plate) Cited by 2Publish Year 2015Author S. RajkhowaDevelopment of multiplex real-time PCR assay for the development of a multiplex pcr assay for detection and(steel) Dec 29, 2014Development of multiplex real-time PCR assay for the detection of Brucella spp., Leptospira spp. and Campylobacter foetus. Selim AM(1), Elhaig MM, Gaede W. Author information (1)Department of Animal Medicine (Infectious Diseases), Faculty of Veterinary Medicine, Benha University, P.O. Box 13736, Toukh, Egypt.

Cited by 3Publish Year 2020Author Privat Ndayihanzamaso, Patrick Karangwa, Diane Mostert, George Mahuku, Guy Blomme, Fento Beed, Rony development of a multiplex pcr assay for detection andDevelopment of a One-Step Multiplex PCR Assay for development of a multiplex pcr assay for detection and

Sep 01, 2017The PCR assay displayed 100% identification agreement with the standard assays. Our multiplex PCR assay is a simple, convenient, and reliable technique for differential identification of MTBC, M. tuberculosis, M. tuberculosis Beijing genotype, and major NTM species.(plate) Cited by 4Publish Year 2017Author Yu Xu, Wanyi Chen, Chunping You, Zhenmin LiuDevelopment of a multiplex PCR assay for detection and development of a multiplex pcr assay for detection and(steel) The developed multiplex PCR assay has similar efficient detection with COB and MPSP PCR, which indicates this multiplex PCR may be a valuable assay for the (plate) Cited by 57Publish Year 2012Author Hongfei Zhu, Quan Wang, Liuqing Wen, Jianguo Xu, Zhujun Shao, Min Chen, Mingliang Chen, Peter R. Ree development of a multiplex pcr assay for detection andDevelopment of a multiplex PCR assay for rapid and development of a multiplex pcr assay for detection and(steel) A multiplex polymerase chain reaction (mPCR) was developed for the simultaneous detection and differentiation of the four genera of fish pathogenic bacteria.

Cited by 8Publish Year 2018Author Vignesh Rathinasamy, Chris Hosking, Lily Tran, Jane Kelley, Genevieve Williamson, Jaclyn Swan, Timot development of a multiplex pcr assay for detection andDevelopment of a Multiplex PCR Assay for Detection of development of a multiplex pcr assay for detection and

Threshold of Multiplex PCR Assay Detection. The assay was performed by first re-suspending cells (EHEC O157:H7 EDL933 or EPEC O127:H6 E2348/69) freshly streaked onto LB agar to an estimated concentration of 4 × 10 9 cells/ml by monitoring the OD 600.Ten-fold serial dilutions were made into sterile distilled water, which was then used as template directly in mPCR reactions.(plate) Development and evaluation of a multiplex PCR assay for development of a multiplex pcr assay for detection and(steel) The multiplex qPCR assay allows for the independent detection of F. hepatica and A. tomentosa DNA using specific primers and a probe targeting the ITS-2 region of the liver fluke or snail. The method allows the highly specific and sensitive (minimal DNA detection levels to 1450 fg) detection of F. hepatica or A. tomentosa .(plate) Development and evaluation of a panel of multiplex one development of a multiplex pcr assay for detection and(steel) Jan 25, 2020Development and evaluation of a panel of multiplex onetube nested real time PCR assay for simultaneous detection of 14 respiratory viruses in five reactions Li Zhao Department of Blood Transfusion, Children's Hospital of Hebei Province, Shijiazhuang, Hebei, China

Development and validation of a real-time multiplex PCR development of a multiplex pcr assay for detection and

The developed qPCR assays demonstrated excellent limit of detection, efficiency, coefficient of determination, analytical and clinical sensitivity and specificity. Conclusion The multiplex qPCR assays were reliable for the diagnosis of onychomycosis, with shorter turn-around time as (plate)Development of a Multiplex PCR Assay for Detection of development of a multiplex pcr assay for detection and(steel) In this study, a multiplex PCR assay was developed for rapid and selective detection of P. fluorescens with biofilm formation ability. The target sequences were 2 genes (adnA and fliC) related to biofilm formation and flagella biosynthesis of P. fluorescens.

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